When he was here, I had to extract genomic DNA from tons of ES clones selected by drug resistance after electroporation. Because he seemed to have done it, I asked him to process that alone. Finally he did well. Because of his help, I could pick 100 ES colonies up at the same time. Otherwise, I would take lone time to do them. Appreciate you, Douguchi.
Taka [Douguchi!!!! Centrifuge all tubes at 12,000rpm for 2min. Move!!!!!!]